Multiplexed IHC Analysis — The Clambey Lab

Important considerations for multiplexed IHC Analysis

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A suggested workflow for multiplexed IHC Analysis

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Perkinelmer RESOURCES for analysis

Phenochart user manual

Inform user manual

Software downloads

phenoptr R package

Our Guides & RESOURCES for analysis

Screen Shot 2019-04-24 at 9.06.41 AM.png — tips for region selection in phenochart

Screen Shot 2019-04-24 at 9.08.04 AM.png — tips for Spectral unmixing & visualization in inform

Screen Shot 2019-04-24 at 10.09.51 AM.png — tips for cell segmentation in inform

Screen Shot 2019-06-20 at 10.56.08 AM.png — txt to fcs conversion R code

Screen Shot 2019-04-24 at 9.08.55 AM.png — cell phenotyping in inform

Screen Shot 2019-04-24 at 9.08.55 AM.png — Cell phenotyping using FlowJo

Screen Shot 2019-06-20 at 10.48.38 AM.png — Cell phenotyping using the tSNE algorithm (Cytobank)

Screen Shot 2019-06-20 at 10.48.38 AM.png — Cell phenotyping using the PhenoGraph algorithm (Cytofkit in R)

Screen Shot 2019-06-20 at 10.43.50 AM.png — IHC Specific analysis using FlowJo

Screen Shot 2019-04-24 at 11.12.10 AM.png — IHC specific analysis using phenoptr (in R)

Screen Shot 2019-06-20 at 10.56.08 AM.png — Alignment of spatial coordinates